Sewell et al. 2026
BDNF Signaling Atlas
An interactive exploration of how 20 distinct spinal cord cell types interpret a single neurotrophic signal. Based on single-cell mass cytometry measuring 19 signaling markers and 18 cell identity markers across 680,466 cells.
Explore Cell Types
Using identity markers and Leiden clustering, we resolved 20 cell populations across a developmental gradient. Each point is a single cell from 680,466 total.
Do all these cell types respond the same way to BDNF? Not even close.
Signaling Response by Cell Type
Each cell type mounts a unique signaling response to BDNF. Mature neurons show 17-fold pERK induction while progenitors barely respond.
Temporal Signaling Dynamics
How does each cell type's response unfold over time? Select a marker to see all 20 clusters' time courses overlaid.
Condition Comparison
How does each cell type respond under different conditions? Compare BDNF stimulation, rescue (CompMed), and deprivation (ContDepr) side by side for any marker.
Is this signaling actually going through TrkB? K252a reveals the answer.
TrkB-Dependence Varies by Cell Type
K252a (a Trk inhibitor) largely abrogates the BDNF response wherever a strong one exists — in mature neurons, and equally in astrocytes and “laden” microglia. Comparing the two conditions shows which responses run through Trk receptors.
Citation
Sewell JM, Bissett AC, Lee G, Zunder ER, Winckler B, Deppmann CD (2026). "A Single-Cell Signaling Atlas of Spinal Cord BDNF Responses Reveals Determinants Beyond Receptor Expression." eLife (Reviewed Preprint). DOI: 10.7554/eLife.111513