Sewell et al. 2026
BDNF Signaling Atlas
An interactive exploration of how 20 distinct spinal cord cell types interpret a single neurotrophic signal. Based on single-cell mass cytometry measuring 18 signaling markers across 680,465 cells.
Explore Cell Types
Leiden clustering of identity markers resolved 20 unique populations across a developmental gradient. Each point is a single cell from 680,465 total.
Do all these cell types respond the same way to BDNF? Not even close.
Signaling Response by Cell Type
Each cell type mounts a unique signaling response to BDNF. Mature neurons show 17-fold pERK induction while progenitors barely respond.
Temporal Signaling Dynamics
How does each cell type's response unfold over time? Select a marker to see all 20 clusters' time courses overlaid.
Condition Comparison
How does each cell type respond under different conditions? Compare BDNF stimulation, rescue (CompMed), and deprivation (ContDepr) side by side for any marker.
Is this signaling actually going through TrkB? K252a reveals the answer.
TrkB-Dependence Varies by Cell Type
K252a (a Trk inhibitor) blocks BDNF signaling in neurons but has little effect on non-neuronal cells, revealing which responses require TrkB.
Citation
Sewell et al. (2026). "Single-cell mass cytometry reveals cell type-specific BDNF signaling in the developing spinal cord." bioRxiv. DOI: 10.64898/2026.03.24.713745